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Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. You can also search for this author in Visiting Scientist, Dept. Stefan Hell and colleagues have developed a reversibly switchable fluorescent protein that can endure more a thousand switching cycles. Stefan W. Hell is a director at both the Max Planck Institute for Biophysical Chemistry in Göttingen and the Max Planck Institute for Medical Research in Heidelberg, Germany.Hell is credited with having conceived, validated and applied the first viable concept for overcoming Abbe’s diffraction-limited resolution barrier in a light-focusing fluorescence microscope. 1997 - 2002. In a data storage task, 25 Grimm's Fairy Tales were coded in ASCII on a 17 × 17-micrometre layer of rsEGFP. & Moerner, W. E. On/off blinking and switching behaviour of single molecules of green fluorescent protein. Okhonin had patented the STED idea. Structure and mechanism of the reversible photoswitch of a fluorescent protein. In the meantime, to ensure continued support, we are displaying the site without styles SW Hell. Westphal, V. et al. Harris, K. M. & Kater, S. B. Dendritic spines: cellular specializations imparting both stability and flexibility to synaptic function. Dendritic spines in living brain slices are super-resolved with about a million times lower light intensities than before.

In 1986, V.A. Direct observation of the nanoscale dynamics of membrane lipids in a living cell. The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage.Get time limited or full article access on ReadCube.Hell, S. W. & Wichmann, J. Copyright: Peter Badge/Typos1 in cop. Zacharias, D. A., Violin, J. D., Newton, A. C. & Tsien, R. Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. A., Sullivan, A. C., Bowman, C. N. & McLeod, R. R. Two-color single-photon photoinitiation and photoinhibition for subdiffraction photolithography. Breaking the diffraction barrier: super-resolution imaging of cells. Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Structural basis for reversible photoswitching in Dronpa. Hell, S. W. Strategy for far-field optical imaging and writing without diffraction limit. Stefan Walter Hell HonFRMS (born 23 December 1962) is a Romanian born German physicist and one of the directors of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany.

All authors discussed the data and commented on the manuscript.The authors declare no competing financial interests.This file contains Supplementary Text and Methods, Supplementary Figures 1-10 with legends, Supplementary Table 1 and Supplementary References. He received the Nobel Prize in Chemistry in 2014 "for the development of super-resolved fluorescence microscopy", together with Eric Betzig and William Moerner. Heintzmann, R., Jovin, T. M. & Cremer, C. Saturated patterned excitation microscopy—a concept for optical resolution improvement. Ando, R., Mizuno, H. & Miyawaki, A. Hopt, A. Eggeling, C. et al. and by a Gottfried-Wilhelm-Leibniz prize of the DFG (to S.W.H. Hell, S. W., Dyba, M. & Jakobs, S. Concepts for nanoscale resolution in fluorescence microscopy. From 1991 to 1993 he worked at the European Molecular Biology Laboratory, followed by stays as a senior researcher at the University of Turku, Finland, between 1993 and 1996, and as a visiting scientist at the University of Oxford, England, in 1994. Internet Explorer). Sign up for the Get the most important science stories of the day, free in your inbox. ).Tim Grotjohann, Ilaria Testa and Marcel Leutenegger: These authors contributed equally to this work.Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, GermanyTim Grotjohann, Ilaria Testa, Marcel Leutenegger, Hannes Bock, Nicolai T. Urban, Flavie Lavoie-Cardinal, Katrin I. Willig, Christian Eggeling, Stefan Jakobs & Stefan W. Hell University of Göttingen Medical School, Robert-Koch-Str. Patterson, G. H., Knobel, S. M., Sharif, W. D., Kain, S. R. & Piston, D. W. Use of the green fluorescent protein and its mutants in quantitative fluorescence microscopy. Bossi, M., Foelling, J., Dyba, M., Westphal, V. & Hell, S. W. Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability. Adam, V. et al. Vats, P. & Rothfield, L. Duplication and segregation of the actin (MreB) cytoskeleton during the prokaryotic cell cycle. Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog. ... (11), 780-782, 1994. For this accomplishment he has received numerous awards, including the 2014 Kavli Prize in Nanoscience and the Nobel Prize in Chemistry.Stefan W. Hell received his doctorate (1990) in physics from the University of Heidelberg.
Brakemann, T. et al. Riedl, J. et al. 1981 - 1987. … Thank you for visiting nature.com. wrote the paper. Li, L., Gattass, R. R., Gershgoren, E., Hwang, H. & Fourkas, J. T. Achieving l/20 resolution by one-color initiation and deactivation of polymerization. Imaging intracellular fluorescent proteins at nanometer resolution. Lifeact: a versatile marker to visualize F-actin. Schwentker, M. A. et al. Single amino acid replacement makes Adam, V. et al. Engineering Science, Oxford University, UK. To obtain Henderson, J. N., Ai, H. W., Campbell, R. E. & Remington, S. J. the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999.